Isolation and characterization of the porcine nuclear factor I (NFI) gene

FEBS Lett. 1988 Aug 15;236(1):27-32. doi: 10.1016/0014-5793(88)80279-5.

Abstract

This study describes the isolation of a major portion of the gene for nuclear factor I (NFI) including its 5'-flanking region with transcriptional start sites. We screened a porcine liver, genomic DNA library in phage EMBL3A with synthetic oligonucleotides derived from tryptic and cyanogen-bromide peptide sequences obtained from purified NFI protein. The NFI gene is present as a single copy in porcine DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Autoradiography
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins*
  • Cloning, Molecular
  • DNA / genetics
  • DNA Restriction Enzymes
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / isolation & purification
  • Endonucleases
  • Exons
  • Genes*
  • Introns
  • Liver / analysis
  • Molecular Sequence Data
  • NFI Transcription Factors
  • Nuclear Proteins
  • Nucleic Acid Hybridization
  • Single-Strand Specific DNA and RNA Endonucleases
  • Transcription Factors*
  • Y-Box-Binding Protein 1

Substances

  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • NFI Transcription Factors
  • Nuclear Proteins
  • Transcription Factors
  • Y-Box-Binding Protein 1
  • YBX1 protein, human
  • DNA
  • Endonucleases
  • DNA Restriction Enzymes
  • Single-Strand Specific DNA and RNA Endonucleases