Abstract
This study describes the isolation of a major portion of the gene for nuclear factor I (NFI) including its 5'-flanking region with transcriptional start sites. We screened a porcine liver, genomic DNA library in phage EMBL3A with synthetic oligonucleotides derived from tryptic and cyanogen-bromide peptide sequences obtained from purified NFI protein. The NFI gene is present as a single copy in porcine DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Autoradiography
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Base Sequence
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CCAAT-Enhancer-Binding Proteins*
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Cloning, Molecular
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DNA / genetics
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DNA Restriction Enzymes
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / isolation & purification
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Endonucleases
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Exons
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Genes*
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Introns
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Liver / analysis
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Molecular Sequence Data
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NFI Transcription Factors
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Nuclear Proteins
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Nucleic Acid Hybridization
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Single-Strand Specific DNA and RNA Endonucleases
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Transcription Factors*
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Y-Box-Binding Protein 1
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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NFI Transcription Factors
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Nuclear Proteins
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Transcription Factors
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Y-Box-Binding Protein 1
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YBX1 protein, human
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DNA
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Endonucleases
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DNA Restriction Enzymes
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Single-Strand Specific DNA and RNA Endonucleases