Effect of Dietary Purified Xanthohumol from Hop (Humulus lupulus L.) Pomace on Adipose Tissue Mass, Fasting Blood Glucose Level, and Lipid Metabolism in KK-Ay Mice

J Oleo Sci. 2017 May 1;66(5):531-541. doi: 10.5650/jos.ess16234. Epub 2017 Apr 13.


We previously showed that xanthohumol-rich hop extract (XRHE, ~18% xanthohumol) exerts anti-obesity effects in rats fed a high-fat diet through regulation of fatty acid metabolism. In this study, we examined the effects of dietary purified xanthohumol from XRHE (PX, ~91.9% xanthohumol) in KK-Ay mice in order to understand the anti-obesity effects of xanthohumol alone because XRHE contains 82% unknown compounds. Dietary consumption of PX significantly inhibited an increase in the visceral fat weight of mice compared to those fed control diet without PX. Plasma leptin level was significantly lower in the PX-fed group than in the control group. Dietary PX lowered hepatic fatty acid synthesis by down-regulation of SREBP1c mRNA expression in the liver. On the other hand, fatty acid β-oxidation in the liver was promoted by dietary PX through the up-regulation of PPARα mRNA expression. Moreover, the fecal levels of fatty acids and carbohydrates increased by dietary PX. PX inhibited lipase or α-amylase activity in vitro. Thus, we found that PX may exert anti-obesity effects through the regulation of lipid metabolism and inhibition of intestinal fat and carbohydrate absorption, and that xanthohumol alone may exert anti-obesity effects.

Keywords: hop; lipid metabolism; mouse; obesity; xanthohumol.

MeSH terms

  • Adipose Tissue / metabolism*
  • Animals
  • Anti-Obesity Agents*
  • Blood Glucose / metabolism*
  • Carbohydrate Metabolism / drug effects
  • Diabetes Mellitus / metabolism*
  • Disease Models, Animal
  • Fasting / blood*
  • Fatty Acids / metabolism
  • Flavonoids / isolation & purification*
  • Flavonoids / pharmacology*
  • Humulus / chemistry*
  • Intestinal Mucosa / metabolism
  • Leptin / blood
  • Lipid Metabolism* / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred Strains
  • Obesity / metabolism*
  • Plant Extracts / isolation & purification*
  • Plant Extracts / pharmacology*
  • Propiophenones / isolation & purification*
  • Propiophenones / pharmacology*
  • Sterol Regulatory Element Binding Protein 1 / metabolism


  • Anti-Obesity Agents
  • Blood Glucose
  • Fatty Acids
  • Flavonoids
  • Leptin
  • Plant Extracts
  • Propiophenones
  • Srebf1 protein, rat
  • Sterol Regulatory Element Binding Protein 1
  • xanthohumol