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. Winter 2017;11(1):14-17.
doi: 10.15171/joddd.2017.003. Epub 2017 Mar 15.

Effect of Nano-Hydroxyapatite Toothpaste on Microhardness Ofartificial Carious Lesions Created on Extracted Teeth

Free PMC article

Effect of Nano-Hydroxyapatite Toothpaste on Microhardness Ofartificial Carious Lesions Created on Extracted Teeth

Asghar Ebadifar et al. J Dent Res Dent Clin Dent Prospects. .
Free PMC article


Background. Many types of toothpastes contain substances that can remineralize initial enamel caries. This study aimed to assess the effect of nano-hydroxyapatite (NHA) on microhardness of artificially created carious lesions. Methods. In this in vitro study, NHA was prepared using sol-gel technique and added to the toothpaste with 7% concentration. A total of 80 extracted sound teeth were collected. The crowns were polished using 500-grit abrasive paper. The specimens were randomly coded from 1 to 80. Number 1 to 40 were assigned to group A and numbers 41 to 80 to group B. The microhardness was measured using HVS-1000 Vickers microhardness tester. The specimens were demineralized using 37% phosphoric acid for 3 minutes in order to create artificial carious lesions and then were rinsed with water, air-sprayed for 3 minutes and dried. Microhardness was measured again. Next, the specimens were brushed for 15 days, twice daily, for 15 seconds. After 15 days, microhardness was measured again. Toothpaste A contained NHA and fluoride and toothpaste B contained fluoride alone. Data were analyzed using SPSS 16, with one-sample Kolmogorov-Smirnov test and ANOVA at a significance level of P<0.05. Results. The microhardness of specimens significantly decreased following acid exposure (P<0.01) but increased again in both groups after exposure to toothpastes. The increase in microhardness was significantly greater in group A (P<0.01). Conclusion. The toothpaste containing NHA was more effective than the toothpaste without NHA for the purpose of remineralization.

Keywords: Toothpaste; decalcification; microhardness; nano-hydroxyapatite; remineralization.

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