Hypotensive acute effect of photobiomodulation therapy on hypertensive rats

Life Sci. 2017 Jun 1:178:56-60. doi: 10.1016/j.lfs.2017.04.011. Epub 2017 Apr 14.


The purpose of this study was to evaluate the acute effect of photobiomodulation therapy (PBM) on arterial pressure in hypertensive and normotensive rats with application in an abdominal region. Normotensive (2K) and hypertensive (2K-1C) wistar rats were treated with PBM. Systolic arterial pressure (SAP), diastolic arterial pressure (DAP), mean arterial pressure (MAP) and heart rate (HR) were measured before, during and after PBM application. The nitric oxide (NO) serum concentration was measured before and after PBM application. Vascular reactivity study was performed in isolated thoracic aortas. Aluminum gallium arsenide (GaAlAs) diode laser was used, at 660nm wavelength and 100mW optical output. The PBM application induced a decrease of SAP in 2K-1C rats. In 2K rats, the PBM application had no effect on SAP, DAP and MAP. Moreover, the magnitude of hypotensive effect was higher in 2K-1C than in 2K rats. The PBM application induced a decrease of HR in 2K-1C and 2K, with higher effect in 2K-1C rats. In 2K-1C, the hypotensive effect induced by PBM was longer than that obtained in 2K rats. PBM application induced an elevation of NO concentration in serum from 2K-1C and 2K rats, with higher effect in 2K-1C. In isolated aortic rings PBM effect is dependent of NO release, and is not dependent of nitric oxide synthase (NOS) activation. Our results indicate that the abdominal acute application of PBM at 660nm is able to induce a long lasting hypotensive effect in hypertensive rats and vasodilation by a NO dependent mechanism.

Keywords: Arterial pressure; Hypertension; Nitric oxide; Photobiomodulation therapy.

MeSH terms

  • Animals
  • Aorta, Thoracic / metabolism
  • Aorta, Thoracic / radiation effects
  • Arterial Pressure / radiation effects*
  • Disease Models, Animal
  • Heart Rate / radiation effects
  • Hypertension / physiopathology
  • Hypertension / radiotherapy*
  • Lasers, Semiconductor / therapeutic use*
  • Low-Level Light Therapy / methods*
  • Male
  • Nitric Oxide / blood*
  • Nitric Oxide Synthase / metabolism
  • Rats
  • Rats, Wistar
  • Time Factors
  • Vasodilation / radiation effects


  • Nitric Oxide
  • Nitric Oxide Synthase