Green listed-a CRISPR screen tool

Sudeepta Kumar Panda; Sanjay V Boddul; Guillermina Yanek Jiménez-Andrade; Long Jiang; Zsolt Kasza; Luciano Fernandez-Ricaud; Fredrik Wermeling

doi:10.1093/bioinformatics/btw739

Green listed-a CRISPR screen tool

Bioinformatics. 2017 Apr 1;33(7):1099-1100. doi: 10.1093/bioinformatics/btw739.

Authors

Sudeepta Kumar Panda¹, Sanjay V Boddul¹, Guillermina Yanek Jiménez-Andrade¹, Long Jiang¹, Zsolt Kasza¹, Luciano Fernandez-Ricaud², Fredrik Wermeling¹

Affiliations

¹ Department of Medicine, Solna, Karolinska Institutet, and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
² Department of Marine Sciences, Lundberg Laboratory, University of Gothenburg, SE-413 90 Gothenburg, Sweden.

PMID: 28414855
DOI: 10.1093/bioinformatics/btw739

Abstract

Motivation: Genome editing using versions of the bacterial CRISPR/Cas9 system can be used to probe the function of selected genes in any organism. Green Listed is a web-based tool that rapidly designs custom CRISPR screens targeting sets of genes defined by the user. It could thus be used to design screens targeting for example all genes differentially expressed during a specific stimuli or all genes related to a specific pathway or function, as well as to generate targeted secondary screens following a large-scale screen.

Availability and implementation: The software, including a demo function as well as explanatory texts and videos, is available through greenlisted.cmm.ki.se .

Contact: fredrik.wermeling@ki.se.

MeSH terms

CRISPR-Cas Systems*
Gene Editing*
Software*