REM sleep deprivation impairs muscle regeneration in rats

Growth Factors. 2017 Feb;35(1):12-18. doi: 10.1080/08977194.2017.1314277. Epub 2017 Apr 17.


Introduction: The aim was observe the influence of sleep deprivation (SD) and sleep recovery on muscle regeneration process in rats submitted to cryolesion.

Methods: Thirty-two Wistar rats were randomly allocated in four groups: control (CTL), SD for 96 h (SD96), control plus sleep recovery period (CTL + R) and SD96h plus 96 h of sleep recovery (SD96 + R). The animals were submitted to muscle injury by cryolesioning, after to SD and sleep recovery.

Results: The major outcomes of this study were the reduction of muscular IGF-1 in both legs (injured and uninjured) and a delay in muscle regeneration process of animals submitted to SD compared to animals that slept, with increase connective tissue, inflammatory infiltrate and minor muscle fibers.

Conclusions: SD impairs muscle regeneration in rats, moreover reduces muscular IGF-1 and sleep recovery was able to restore it to basal levels, but it was not enough to normalize the muscle regeneration.

Keywords: IGF-1; muscle recovery; skeletal muscle; sleep loss.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Insulin-Like Growth Factor I / metabolism
  • Male
  • Muscle, Skeletal / injuries
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / physiology*
  • Rats
  • Rats, Wistar
  • Regeneration*
  • Sleep Deprivation / physiopathology*
  • Sleep, REM*


  • Insulin-Like Growth Factor I