An HLA-B7-specific antibody in an HLA-B*07 positive patient explained by a nonexpressed allele (HLA-B*07:181N)

HLA. 2017 Jul;90(1):32-35. doi: 10.1111/tan.13045. Epub 2017 Apr 17.

Abstract

Antibody identification by a bead array assay in a kidney patient revealed several HLA-specific antibodies including one directed against the HLA-B7 antigen. Low-resolution typing of the patient indicated the presence of an HLA-B*07 allele. To rule out an HLA-specific autoantibody the HLA-typing of the patient was further refined by nucleotide sequencing on a next-generation sequencing platform and eventually showed an HLA-B*39:01:01:03 and HLA-B*07:181N genotype. Thereby the allospecific nature of the antibody was proven. The HLA-B7-specific antibody could be explained by an immunization during the first kidney-transplantation in 1996 with an HLA-B*07 positive donor. When assessing the plausibility of antibodies, the presence of nonexpressed alleles should be taken into consideration.

Keywords: HLA-B7; bead array test; next generation sequencing; null alleles; sequencing based typing.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Alleles*
  • Base Sequence
  • Female
  • Gene Expression
  • Genotype
  • HLA-B39 Antigen / genetics*
  • HLA-B39 Antigen / immunology
  • HLA-B7 Antigen / genetics*
  • HLA-B7 Antigen / immunology
  • High-Throughput Nucleotide Sequencing
  • Histocompatibility Testing
  • Humans
  • Isoantibodies / genetics*
  • Kidney Transplantation / methods*
  • Polymerase Chain Reaction
  • Protein Isoforms / genetics
  • Protein Isoforms / immunology
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Tissue Donors
  • Transplant Recipients*
  • Transplantation, Homologous

Substances

  • HLA-B*07 antigen
  • HLA-B39 Antigen
  • HLA-B7 Antigen
  • Isoantibodies
  • Protein Isoforms