Detection of Urine Metabolites in a Rat Model of Chronic Fatigue Syndrome before and after Exercise

Biomed Res Int. 2017;2017:8182020. doi: 10.1155/2017/8182020. Epub 2017 Mar 22.

Abstract

Purpose. The aim of the present study was to elucidate the metabolic mechanisms associated with chronic fatigue syndrome (CFS) via an analysis of urine metabolites prior to and following exercise in a rat model. Methods. A rat model of CFS was established using restraint-stress, forced exercise, and crowded and noisy environments over a period of 4 weeks. Behavioral experiments were conducted in order to evaluate the model. Urine metabolites were analyzed via gas chromatography-mass spectrometry (GC-MS) in combination with multivariate statistical analysis before and after exercise. Results. A total of 20 metabolites were detected in CFS rats before and after exercise. Three metabolic pathways (TCA cycle; alanine, aspartate, and glutamate metabolism; steroid hormone biosynthesis) were significantly impacted before and after exercise, while sphingolipid metabolism alone exhibited significant alterations after exercise only. Conclusion. In addition to metabolic disturbances involving some energy substances, alterations in steroid hormone biosynthesis and sphingolipid metabolism were detected in CFS rats. Sphingosine and 21-hydroxypregnenolone may be key biomarkers of CFS, potentially offering evidence in support of immune dysfunction and hypothalamic-pituitary-adrenal (HPA) axis hypoactivity in patients with CFS.

MeSH terms

  • 17-alpha-Hydroxypregnenolone / urine*
  • Amino Acids / metabolism
  • Animals
  • Behavior, Animal
  • Disease Models, Animal
  • Fatigue Syndrome, Chronic / physiopathology
  • Fatigue Syndrome, Chronic / urine*
  • Female
  • Gas Chromatography-Mass Spectrometry / methods
  • Gonadal Steroid Hormones / metabolism
  • Humans
  • Physical Conditioning, Animal*
  • Rats
  • Rats, Sprague-Dawley
  • Sphingosine / urine*

Substances

  • Amino Acids
  • Gonadal Steroid Hormones
  • 17-alpha-Hydroxypregnenolone
  • 21-hydroxypregnenolone
  • Sphingosine