Superoxide production from nonenzymatically glycated protein

FEBS Lett. 1988 Aug 29;236(2):406-10. doi: 10.1016/0014-5793(88)80066-8.

Abstract

Nonenzymatically glycated human serum albumin and glycated poly-lysine(Lys) in vitro brought about the reduction of nitroblue tetrazolium and ferricytochrome c at pH 9.06 and pH 7.8, respectively. This reduction was inhibited partially by superoxide dismutase (SOD). Glycated poly-Lys caused the oxidation of NADH in the presence of LDH at pH 7.0 which was completely inhibited by SOD. Glycated material was found to function both as a reductant and an oxidant. The reactivity of glycated material is discussed and a possible mechanism by which superoxide is produced is proposed. Results may give a clue to diabetic complications.

MeSH terms

  • Cytochrome c Group / metabolism
  • Glycosylation*
  • In Vitro Techniques
  • Luminescence
  • Nitroblue Tetrazolium / metabolism
  • Oxidation-Reduction
  • Polylysine / metabolism*
  • Serum Albumin / metabolism*
  • Sulfhydryl Compounds
  • Superoxides / metabolism*

Substances

  • Cytochrome c Group
  • Serum Albumin
  • Sulfhydryl Compounds
  • glycosylated serum albumin
  • Superoxides
  • Polylysine
  • Nitroblue Tetrazolium