Autoantibody biomarkers for the detection of serous ovarian cancer

Gynecol Oncol. 2017 Jul;146(1):129-136. doi: 10.1016/j.ygyno.2017.04.005. Epub 2017 Apr 18.


Objective The purpose of this study was to identify a panel of novel serum tumor antigen-associated autoantibody (TAAb) biomarkers for the diagnosis of high-grade serous ovarian cancer.

Methods: To detect TAAb we probed high-density programmable protein microarrays (NAPPA) containing 10,247 antigens with sera from patients with serous ovarian cancer (n=30 cases/30 healthy controls) and measured bound IgG. We identified 735 promising tumor antigens and evaluated these with an independent set of serous ovarian cancer sera (n=30 cases/30 benign disease controls/30 healthy controls). Thirty-nine potential tumor autoantigens were identified and evaluated using an orthogonal programmable ELISA platform against a total of 153 sera samples (n=63 cases/30 benign disease controls/60 healthy controls). Sensitivities at 95% specificity were calculated and a classifier for the detection of high-grade serous ovarian cancer was constructed.

Results: We identified 11-TAAbs (ICAM3, CTAG2, p53, STYXL1, PVR, POMC, NUDT11, TRIM39, UHMK1, KSR1, and NXF3) that distinguished high-grade serous ovarian cancer cases from healthy controls with a combined 45% sensitivity at 98% specificity.

Conclusion: These are potential circulating biomarkers for the detection of serous ovarian cancer, and warrant confirmation in larger clinical cohorts.

Keywords: Autoantibody; Biomarker; Diagnostics; Ovarian cancer; Proteomics.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antibodies, Neoplasm / blood*
  • Antibodies, Neoplasm / immunology
  • Antigens, Neoplasm / blood
  • Antigens, Neoplasm / immunology
  • Autoantibodies / blood*
  • Autoantibodies / immunology
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / immunology*
  • Case-Control Studies
  • Cystadenocarcinoma, Serous / blood
  • Cystadenocarcinoma, Serous / immunology*
  • Female
  • Humans
  • Middle Aged
  • Ovarian Neoplasms / blood
  • Ovarian Neoplasms / immunology*
  • Protein Array Analysis


  • Antibodies, Neoplasm
  • Antigens, Neoplasm
  • Autoantibodies
  • Biomarkers, Tumor