Optimized CUBIC protocol for three-dimensional imaging of chicken embryos at single-cell resolution

Development. 2017 Jun 1;144(11):2092-2097. doi: 10.1242/dev.145805. Epub 2017 Apr 21.

Abstract

The CUBIC tissue-clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light-sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell resolution in whole early-stage chicken embryos and in the whole brains of late-stage embryos.

Keywords: 3D imaging; Chicken embryo; Light-sheet microscopy; Tissue clearing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / metabolism
  • Benzoates / chemistry
  • Benzyl Alcohol / chemistry
  • Brain / cytology*
  • Brain / embryology*
  • Chick Embryo
  • Imaging, Three-Dimensional / methods*
  • Lasers
  • Microscopy, Confocal
  • Single-Cell Analysis / methods*

Substances

  • Antibodies
  • Benzoates
  • Benzyl Alcohol
  • benzyl benzoate