The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: cell-free Ty1 transposition

Cell. 1988 Sep 23;54(7):955-66. doi: 10.1016/0092-8674(88)90110-9.


Yeast Ty1 elements are retrotransposons that transpose via an RNA intermediate found in a virus-like particle (Ty-VLP). A Ty-encoded reverse transcriptase activity found inside the particles is capable of giving rise to full-length reverse transcripts. The predominant form of these reverse transcripts is a full-length linear duplex DNA. We have developed a cell-free system for transposition of Ty1 DNA molecules into a bacteriophage lambda target. Purified Ty-VLPs and target DNA are the only macromolecular components required for the transposition reaction. A TYB-encoded protein, p90-TYB, contains amino acid sequences that are similar to those of retroviral integrase proteins. Mutations in the integrase coding region abolish transposition both in vivo and in vitro.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / biosynthesis
  • Bacteriophage lambda / genetics
  • Base Sequence
  • Cell Fractionation
  • Cell-Free System
  • Centrifugation, Density Gradient
  • DNA Nucleotidyltransferases / genetics
  • DNA Transposable Elements*
  • DNA, Circular / isolation & purification
  • DNA, Fungal / genetics*
  • DNA, Fungal / isolation & purification
  • DNA, Recombinant / isolation & purification
  • Escherichia coli / genetics
  • Integrases
  • Mutation
  • Plasmids
  • Retroviridae / genetics
  • Saccharomyces cerevisiae / genetics*
  • Sequence Homology, Nucleic Acid


  • Antibodies, Monoclonal
  • DNA Transposable Elements
  • DNA, Circular
  • DNA, Fungal
  • DNA, Recombinant
  • DNA Nucleotidyltransferases
  • Integrases