It has been demonstrated that circulating MicroRNAs (miRNAs) could be potential biomarkers for cancer diagnosis and prognosis. For pancreatic cancer (PCa), little is known about miR-221-3p biological function or its prognostic value. In the current study, we profiled miR-221-3p expression in PCa cell lines. Compared with normal pancreases ductal epithelial cells, miR-221-3p is up-regulated in all PCa cell lines analysed. In SW1990 cells, overexpression of miR-221-3p increased cell proliferation and inhibited apoptosis, while inhibition of miR-221-3p decreased cell growth rate and promoted apoptosis. Compared with adjacent non-tumour tissues, miR-221-3p was up-regulated in all 21 PCa tissues. Expression level of miR-221-3p was investigated in plasma and statistical analyses showed that circulating miR-221-3p expression level was correlated with distant metastasis and TNM stages. The receiver-operating characteristic (ROC) curves and the area under the ROC curve (AUC) suggested that the diagnostic efficacy for distant metastasis of miR-221-3p is better than CA19-9 (AUC: 0.689 vs. 0.587). To summary, we found miR-221-3p could promote cell proliferation and inhibit apoptosis in PCa cells and circulating miR-221-3p could serve as a biomarker for PCa.
Keywords: Plasma; miR-221-3p; pancreatic cancer; survival.