Evaluation of the protective effect of Botulinum toxin type A injection on otitis media with effusion

Acta Otolaryngol. 2017 Sep;137(9):917-922. doi: 10.1080/00016489.2017.1314008. Epub 2017 Apr 22.


Objective: This study makes an analysis of the effect of Botulinum toxin type A on otitis media with effusion in rats.

Method: As part of the study, 24 male Wistar Albino rats were divided into three groups: Group 1: Botulinum toxin Type A and Histamıne (intratympanic 0.2 ml = 20 unit BTA); Group 2: Saline and Histamine (intratympanic 0.2 ml 0.9%); Group 3: Histamine (intratympanic 0.2 ml). Histamine (intratympanic 0.2 ml) was administered into the right ear for all groups. DPOAE and ABR tests were carried out on days 5, 10, 15 and 20 from the beginning of the study.

Results: There was no significant difference between DPOAE and ABR scores of the groups. In each group, the DPOAE scores for the right ear significantly decreased on day 5 when compared to the basal scores. In each group, there was no significant difference between days 5, 10 and 15 for the right ear.

Conclusions: Botulinum toxin type A blocked the secretion of glands in the middle ear and showed no effect to prevent the formation of serous otitis. In addition, it was found out that Botulinum toxin did not lead to any morphological change in the cochlea.

Keywords: Botulinum toxin; hearing loss; otitis media; rat; serous otitis media.

MeSH terms

  • Acetylcholine Release Inhibitors / pharmacology
  • Acetylcholine Release Inhibitors / therapeutic use*
  • Animals
  • Botulinum Toxins, Type A / pharmacology
  • Botulinum Toxins, Type A / therapeutic use*
  • Drug Evaluation, Preclinical
  • Evoked Potentials, Auditory, Brain Stem
  • Male
  • Otitis Media with Effusion / drug therapy*
  • Rats, Wistar


  • Acetylcholine Release Inhibitors
  • Botulinum Toxins, Type A