Convenient PCR method for variable-number tandem-repeat typing of Mycobacterium tuberculosis clinical isolates

J Microbiol Methods. 2017 Aug;139:12-14. doi: 10.1016/j.mimet.2017.04.008. Epub 2017 Apr 22.


Variable-number tandem-repeat typing for Mycobacterium tuberculosis clinical isolates contributes to evidence-based tuberculosis control. However, cumbersome PCR procedures for the typing have disturbed routine analyses. We proposed a convenient PCR method for the typing using a PCR master mix that provides rapidity and long-term stability of the frozen PCR cocktail.

Keywords: Frozen PCR cocktail; High labor effectiveness; Human error reduction; Rapidity; SapphireAmp Fast Master Mix.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial / genetics
  • Humans
  • Indicators and Reagents
  • Minisatellite Repeats*
  • Molecular Typing* / methods
  • Mycobacterium tuberculosis / classification*
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Polymerase Chain Reaction / methods*
  • Time Factors
  • Tuberculosis / microbiology


  • DNA, Bacterial
  • Indicators and Reagents