The Use of Proteomics in Assisted Reproduction

In Vivo. 2017 May-Jun;31(3):267-283. doi: 10.21873/invivo.11056.


Despite the explosive increase in the use of Assisted Reproductive Technologies (ART) over the last 30 years, their success rates remain suboptimal. Proteomics is a rapidly-evolving technology-driven science that has already been widely applied in the exploration of human reproduction and fertility, providing useful insights into its physiology and leading to the identification of numerous proteins that may be potential biomarkers and/or treatment targets of a successful ART pregnancy. Here we present a brief overview of the techniques used in proteomic analyses and attempt a comprehensive presentation of recent data from mass spectrometry-based proteomic studies in humans, regarding all components of ARTs, including the male and female gamete, the derived zygote and embryo, the endometrium and, finally, the ART offspring both pre- and postnatally.

Keywords: ICSI; IVF; Proteomics; assisted reproduction; embryo; epigenetics; fertility; offspring health; oocyte; review; sperm.

Publication types

  • Review

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Humans
  • Proteome / metabolism*
  • Proteomics / methods
  • Reproduction / physiology*
  • Reproductive Techniques, Assisted


  • Biomarkers
  • Proteome