Endocrine network essential for reproductive success in Drosophila melanogaster

Proc Natl Acad Sci U S A. 2017 May 9;114(19):E3849-E3858. doi: 10.1073/pnas.1620760114. Epub 2017 Apr 24.


Ecdysis-triggering hormone (ETH) was originally discovered and characterized as a molt termination signal in insects through its regulation of the ecdysis sequence. Here we report that ETH persists in adult Drosophila melanogaster, where it functions as an obligatory allatotropin to promote juvenile hormone (JH) production and reproduction. ETH signaling deficits lead to sharply reduced JH levels and consequent reductions of ovary size, egg production, and yolk deposition in mature oocytes. Expression of ETH and ETH receptor genes is in turn dependent on ecdysone (20E). Furthermore, 20E receptor knockdown specifically in Inka cells reduces fecundity. Our findings indicate that the canonical developmental roles of 20E, ETH, and JH during juvenile stages are repurposed to function as an endocrine network essential for reproductive success.

Keywords: ecdysis triggering hormone; ecdysone; fecundity; juvenile hormone; oogenesis.

MeSH terms

  • Animals
  • Drosophila melanogaster
  • Endocrine System / metabolism*
  • Female
  • Insect Hormones / genetics
  • Insect Hormones / metabolism*
  • Juvenile Hormones / genetics
  • Juvenile Hormones / metabolism
  • Male
  • Neuropeptides / genetics
  • Neuropeptides / metabolism
  • Receptors, Peptide / genetics
  • Receptors, Peptide / metabolism*
  • Reproduction / physiology
  • Signal Transduction / physiology*


  • Insect Hormones
  • Juvenile Hormones
  • Neuropeptides
  • Receptors, Peptide
  • ecdysis-triggering hormone receptor, Drosophila
  • ecdysis-triggering hormone, Drosophila
  • allatotropin