Der f 35: An MD-2-like house dust mite allergen that cross-reacts with Der f 2 and Pso o 2

Allergy. 2017 Nov;72(11):1728-1736. doi: 10.1111/all.13192. Epub 2017 May 23.

Abstract

Background: Dermatophagoides farinae is a source of airborne house dust mite (HDM) allergens. We elucidated IgE-reactive allergens from D. farinae by two-dimensional immunoblotting-based allergenome analysis, and identified one new allergen, named Der f 35, that possesses IgE-binding capacity comparable to that of Der f 2. The aim of this study was to clarify the allergenic capacity of new HDM allergen Der f 35.

Methods: We cloned der f 35 from D. farinae mRNA and produced recombinant Der f 35 in Escherichia coli. The IgE-binding capacity of Der f 35 and its cross-reactivity with group 2 allergens from D. farinae and Psoroptes ovis were determined by enzyme-linked immunosorbent assay (ELISA) and ELISA inhibition assays, respectively.

Results: The deduced amino acid sequence for der f 35, which possesses the MD-2-related lipid-recognition domain, showed higher identity with group 2 allergens from P. ovis (61.5%) and Blomia tropicalis (50.7%) than with Der f 2 (40.8%). Der f 35 showed IgE-binding frequencies of 77.5% (31/40) for the native form upon allergenome analysis and 51.4% (18/35) for recombinant structure by ELISA. Der f 35 showed cross-reactivity with Der f 2 and Pso o 2 in reaction with HDM-allergic patients' IgE by ELISA inhibition assay.

Conclusion: Der f 35 is a candidate major allergen from D. farinae, which is more similar to group 2 allergens from sheep scab mite and storage mites. Der f 35 could be responsible for the cross-reactivity among group 2 mite allergens.

Keywords: Dermatophagoides farinae; Der f 2; allergen; group 2 allergen; house dust mite.

MeSH terms

  • Allergens / immunology*
  • Animals
  • Antigens, Dermatophagoides / immunology*
  • Arthropod Proteins / immunology
  • Cross Reactions / immunology*
  • Dermatophagoides farinae / immunology
  • Immunoglobulin E / metabolism
  • Mites / immunology*
  • Psoroptidae / immunology
  • Sequence Analysis, Protein
  • Sheep

Substances

  • Allergens
  • Antigens, Dermatophagoides
  • Arthropod Proteins
  • Dermatophagoides farinae antigen f 2
  • Immunoglobulin E

Associated data

  • GENBANK/LC175222
  • GENBANK/D10448
  • GENBANK/AM263560
  • GENBANK/EX1663
  • GENBANK/AF047613
  • GENBANK/AY288141
  • GENBANK/AJ272216
  • GENBANK/AY497897
  • GENBANK/EU106610
  • GENBANK/EU106609
  • GENBANK/DQ367850
  • GENBANK/IPR003172
  • GENBANK/Q965E2
  • GENBANK/X83875
  • GENBANK/Y12690
  • GENBANK/EX163551
  • GENBANK/AY288140
  • GENBANK/AY288139
  • GENBANK/X81399
  • GENBANK/AJ487972
  • GENBANK/AJ487973
  • GENBANK/AJ249864
  • GENBANK/AF276239
  • GENBANK/FM177223
  • GENBANK/D10447
  • GENBANK/D10449
  • GENBANK/AY066008
  • GENBANK/AJ862836
  • GENBANK/AJ862837
  • GENBANK/AJ862838
  • GENBANK/DQ185511
  • GENBANK/AY283288
  • GENBANK/EF205129
  • GENBANK/AF047614
  • GENBANK/DQ643992