Automated and Manual Patch Clamp Data of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons

Sci Data. 2017 Apr 25;4:170056. doi: 10.1038/sdata.2017.56.

Abstract

Human induced pluripotent stem cells can be differentiated into dopaminergic neurons (Dopa.4U). Dopa.4U neurons expressed voltage-gated NaV and KV channels and showed neuron-like spontaneous electrical activity. In automated patch clamp measurements with suspended Dopa.4U neurons, delayed rectifier K+ current (delayed KV) and rapidly inactivating A-type K+ current (fast KV) were identified. Examination of the fast KV current with inhibitors yielded IC50 values of 0.4 mM (4-aminopyridine) and 0.1 mM (tetraethylammonium). In manual patch clamp measurements with adherent Dopa.4U neurons, fast KV current could not be detected, while the delayed KV current showed an IC50 of 2 mM for 4-aminopyridine. The NaV channels in adherent and suspended Dopa.4U neurons showed IC50 values for tetrodotoxin of 27 and 2.9 nM, respectively. GABA-induced currents that could be observed in adherent Dopa.4U neurons could not be detected in suspended cells. Application of current pulses induced action potentials in approx. 70 % of the cells. Our results proved the feasibility of automated electrophysiological characterization of neuronal cells.

Publication types

  • Dataset
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Dopaminergic Neurons / cytology
  • Dopaminergic Neurons / physiology*
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / physiology*
  • Ion Channels
  • Patch-Clamp Techniques
  • Potassium Channels / physiology*

Substances

  • Ion Channels
  • Potassium Channels

Associated data

  • figshare/10.6084/m9.figshare.c.3674959