Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis

Biochimie. 1988 Apr;70(4):559-66. doi: 10.1016/0300-9084(88)90093-4.

Abstract

Cloning vector plasmids have been constructed on the basis of the broad host range plasmid pAM beta 1 and used for the cloning of a nisin resistance determinant in Streptococcus lactis. They incorporate several desirable features for gene cloning in S. lactis and other transformable Gram-positive bacteria. They carry an easily selectable erythromycin resistance marker, are present at low (6-9) or high (45-85) copy number in S. lactis and possess a convenient polyrestriction site sequence. A significant advantage of these plasmids is their capability to carry and stably maintain very large cloned DNA fragments (up to 30 kilobases).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics
  • DNA, Recombinant
  • Drug Resistance, Microbial / genetics
  • Erythromycin
  • Genes, Bacterial
  • Genetic Vectors*
  • Lactococcus lactis / genetics*
  • Nisin
  • Plasmids*
  • Transformation, Bacterial

Substances

  • DNA, Bacterial
  • DNA, Recombinant
  • Nisin
  • Erythromycin
  • DNA Restriction Enzymes