Nuclear survivin expression correlates with endoglin-assessed microvascularisation in laryngeal carcinoma

J Clin Pathol. 2017 Dec;70(12):1033-1037. doi: 10.1136/jclinpath-2016-204230. Epub 2017 Apr 26.

Abstract

Aims: Survivin-a member of the family of inhibitor of apoptosis proteins that control cell division, apoptosis and metastasis-is overexpressed in virtually all human cancers, including laryngeal squamous cell carcinoma (LSCC). Recent findings also correlate survivin expression with the regulation of angiogenesis. The novel main aim of this study was a preliminary investigation into the potential role of survivin expression in LSCC neoangiogenesis, as determined by endoglin-assessed microvascular density (MVD).

Methods: Immunohistochemical expression of nuclear survivin and endoglin-assessed MVD were ascertained by image analysis in 75 consecutive LSCCs.

Results: Statistical analysis disclosed a strong direct correlation between nuclear survivin expression and MVD. Patients whose nuclear survivin expression was ≥6.0% had a significantly higher LSCC recurrence rate, and a significantly shorter disease-free survival (DFS) than those with a nuclear survivin expression <6.0%. The LSCC recurrence rate was also higher and the DFS shorter in patients with endoglin-assessed MVD ≥6.89%. The OR for recurrence was 2.79 in patients with LSCC with a nuclear survivin expression ≥6.0%, and 12.31 in those with an MVD≥6.89%.

Conclusions: Survivin-targeting strategies to enhance tumour cell response to apoptosis and inhibit tumour growth should receive more attention with a view to developing agents for use in multimodality advanced LSCC treatment, or combined with conventional chemotherapy. Given the present preliminary evidence in LSCC, survivin targeting should also be further investigated for anti-angiogenic purposes, to reduce tumour blood flow and induce cancer necrosis.

Keywords: ANGIOGENESIS; CARCINOMA; LARYNX.

MeSH terms

  • Aged
  • Biomarkers, Tumor / analysis*
  • Carcinoma, Squamous Cell / chemistry*
  • Carcinoma, Squamous Cell / mortality
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / surgery
  • Cell Nucleus / chemistry*
  • Cell Nucleus / pathology
  • Disease-Free Survival
  • Endoglin / analysis*
  • Female
  • Head and Neck Neoplasms / chemistry*
  • Head and Neck Neoplasms / mortality
  • Head and Neck Neoplasms / pathology
  • Head and Neck Neoplasms / surgery
  • Humans
  • Immunohistochemistry
  • Inhibitor of Apoptosis Proteins / analysis*
  • Kaplan-Meier Estimate
  • Laryngeal Neoplasms / chemistry*
  • Laryngeal Neoplasms / mortality
  • Laryngeal Neoplasms / pathology
  • Laryngeal Neoplasms / surgery
  • Male
  • Microvessels / chemistry*
  • Microvessels / pathology
  • Middle Aged
  • Neoplasm Recurrence, Local
  • Odds Ratio
  • Risk Factors
  • Squamous Cell Carcinoma of Head and Neck
  • Survivin
  • Time Factors

Substances

  • BIRC5 protein, human
  • Biomarkers, Tumor
  • ENG protein, human
  • Endoglin
  • Inhibitor of Apoptosis Proteins
  • Survivin