Epidural focal brain cooling abolishes neocortical seizures in cats and non-human primates

Neurosci Res. 2017 Sep;122:35-44. doi: 10.1016/j.neures.2017.04.007. Epub 2017 Apr 24.


Focal brain cooling (FBC) is under investigation in preclinical trials of intractable epilepsy (IE), including status epilepticus (SE). This method has been studied in rodents as a possible treatment for epileptic disorders, but more evidence from large animal studies is required. To provide evidence that FBC is a safe and effective therapy for IE, we investigated if FBC using a titanium cooling plate can reduce or terminate focal neocortical seizures without having a significant impact on brain tissue. Two cats and two macaque monkeys were chronically implanted with an epidural FBC device over the somatosensory and motor cortex. Penicillin G was delivered via the intracranial cannula for induction of local seizures. Repetitive FBC was performed using a cooling device implanted for a medium-term period (FBC for 30min at least twice every week; 3 months total) in three of the four animals. The animals exhibited seizures with repetitive epileptiform discharges (EDs) after administration of penicillin G, and these discharges decreased at less than 20°C cooling with no adverse histological effects. The results of this study suggest that epidural FBC is a safe and effective potential treatment for IE and SE.

Keywords: Epilepsy; Focal brain cooling; Hypothermia; Implantable device; Motor cortex; Neuromodulation; Penicillin G.

MeSH terms

  • Animals
  • Cats
  • Disease Models, Animal
  • Electrocorticography
  • Epilepsy / chemically induced
  • Epilepsy / physiopathology
  • Epilepsy / therapy*
  • Female
  • Hypothermia, Induced* / adverse effects
  • Hypothermia, Induced* / instrumentation
  • Hypothermia, Induced* / methods
  • Macaca
  • Male
  • Motor Cortex / physiopathology*