Diagnostic Biomarkers of Alzheimer's Disease as Identified in Saliva using 1H NMR-Based Metabolomics

J Alzheimers Dis. 2017;58(2):355-359. doi: 10.3233/JAD-161226.


Using 1H NMR metabolomics, we biochemically profiled saliva samples collected from healthy-controls (n = 12), mild cognitive impairment (MCI) sufferers (n = 8), and Alzheimer's disease (AD) patients (n = 9). We accurately identified significant concentration changes in 22 metabolites in the saliva of MCI and AD patients compared to controls. This pilot study demonstrates the potential for using metabolomics and saliva for the early diagnosis of AD. Given the ease and convenience of collecting saliva, the development of accurate and sensitive salivary biomarkers would be ideal for screening those at greatest risk of developing AD.

Keywords: 1H NMR; Alzheimer’s disease; metabolomics; mild cognitive impairment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / diagnosis*
  • Alzheimer Disease / metabolism
  • Cognitive Dysfunction / diagnosis*
  • Cognitive Dysfunction / metabolism
  • Early Diagnosis
  • Female
  • Humans
  • Logistic Models
  • Male
  • Mental Status Schedule
  • Metabolomics / methods*
  • Proton Magnetic Resonance Spectroscopy / methods*
  • ROC Curve
  • Saliva / metabolism*