Multiplexed Point-of-Care Testing - xPOCT

Trends Biotechnol. 2017 Aug;35(8):728-742. doi: 10.1016/j.tibtech.2017.03.013. Epub 2017 Apr 26.

Abstract

Multiplexed point-of-care testing (xPOCT), which is simultaneous on-site detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resource-limited settings (such as in the developing world, in doctors' offices, or directly at home). Nevertheless, only single-analyte approaches are typically considered as the major paradigm in many reviews of point-of-care testing. Here, we comprehensively review the present diagnostic systems and techniques for xPOCT applications. Different multiplexing technologies (e.g., bead- or array-based systems) are considered along with their detection methods (e.g., electrochemical or optical). We also address the unmet needs and challenges of xPOCT. Finally, we critically summarize the in-field applicability and the future perspectives of the presented approaches.

Keywords: lab-on-a-chip (LOC); microfluidics; multianalyte analysis; multiplexing; on-site testing; point-of-care testing (POCT).

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Humans
  • Lab-On-A-Chip Devices / trends*
  • Microfluidic Analytical Techniques* / instrumentation
  • Microfluidic Analytical Techniques* / methods
  • Microfluidic Analytical Techniques* / trends
  • Point-of-Care Systems / trends*