6 HCV Genotyping 9G test for HCV 1a, 1b, 2, 3, 4 and 6 (6a, 6f, 6i and 6n) with high accuracy

J Virol Methods. 2017 Aug:246:95-99. doi: 10.1016/j.jviromet.2017.04.017. Epub 2017 Apr 27.

Abstract

According to EASL guidelines and WHO recommendations, the accurate detection of HCV genotypes such as HCV 1a, HCV1b, HCV 2, HCV 3, HCV 4, and HCV 6 (6a, 6f, 6i, 6n) is crucial for the efficient treatment of hepatitis C. HCV Genotyping 9G test allows simultaneous genotyping of HCV 1a, 1b, 2, 3, 4, and 6 (6a, 6f, 6i, and 6n) in clinical samples in 30min. The performance of the test was evaluated by comparison with sequence analysis. Serum samples (n=152) from HCV-infected patients (n=110) and healthy individuals (n=42) were processed under blinded codes. The k coefficient (kappa) values indicated high agreement between the HCV Genotyping 9G test and sequencing. The sensitivity and specificity of the test were 99.1% and 99.7%, respectively. The results indicate that HCV Genotyping 9G test is rapid, reliable, sensitive, and accurate for screening and genotyping of HCV in the clinical specimens.

Keywords: HCV; HCV-Genotyping; Hepatitis C; Liver cirrhosis; Sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers
  • Genotype
  • Genotyping Techniques / methods*
  • Hepacivirus / classification
  • Hepacivirus / genetics*
  • Humans
  • Liver Cirrhosis / virology
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Viral Nonstructural Proteins / genetics

Substances

  • DNA Primers
  • Viral Nonstructural Proteins