Monitoring of Atg5-Independent Mitophagy

Methods Mol Biol. 2018:1759:125-132. doi: 10.1007/7651_2017_16.

Abstract

Mitophagy is a mitochondrial quality control mechanism where damaged and surplus mitochondria are removed by autophagy. There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the erythrocyte mitophagy. In this chapter we describe the various experimental approaches to assess Atg5-indepedndent mitophagy in mammalian cells.

Keywords: Atg5-independent autophagy; Erythrocyte maturation; Mitophagy; Parkin; Selective autophagy.

MeSH terms

  • Animals
  • Autophagy-Related Protein 5 / genetics
  • Autophagy-Related Protein 5 / metabolism*
  • Biological Assay
  • Cell Line
  • Erythrocytes / metabolism
  • Flow Cytometry
  • Gene Knockout Techniques
  • Genes, Reporter
  • Humans
  • Mice
  • Mitochondria / genetics
  • Mitochondria / metabolism*
  • Mitochondria / ultrastructure
  • Mitophagy*
  • Sequence Deletion
  • Signal Transduction*

Substances

  • Autophagy-Related Protein 5