Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry

Methods Mol Biol. 2017;1594:191-203. doi: 10.1007/978-1-4939-6934-0_12.

Abstract

Flow cytometry is a method that allows high throughput analysis of individual cells in suspension. By inclusion of fluorescently labelled antibodies, it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.

Keywords: Flow cytometry; Granule; Lysosomal exocytosis; Lysosomal-associated membrane protein-1 (LAMP-1); Lysosome; Platelet; Platelet activation; Platelet secretion.

MeSH terms

  • Blood Platelets / metabolism
  • Exocytosis / physiology*
  • Flow Cytometry
  • Humans
  • Lysosomal-Associated Membrane Protein 1 / metabolism
  • Lysosomes / metabolism*

Substances

  • Lysosomal-Associated Membrane Protein 1