Molecular effects of hesperetin, a citrus flavanone on7,12-dimethylbenz(a)anthracene induced buccal pouch squamous cell carcinoma in golden Syrian hamsters

Arch Physiol Biochem. 2017 Oct;123(4):265-278. doi: 10.1080/13813455.2017.1317815. Epub 2017 May 1.


In recent years, researchers have been focused on citrus flavanone, a naturally occurring bioactive substance of hesperetin. To investigate the molecular mechanism based chemopreventive efficacy of hesperetin on 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch (HBP) squamous cell carcinoma (SCC). The oral tumour was provoked by painted with 0.5% DMBA on left buccal pouch thrice a week for 10 consecutive weeks developed well-differentiated SCC and tumour formation was 100% in DMBA alone. We evaluated the chemopreventive potential of hesperetin by assessing the lipid peroxidation (LPO) by-products, status of enzymatic, non-enzymatic antioxidants, detoxifying agents etc. Moreover, modulating expression of apoptotic and cell proliferation markers were observed in HBP SCC experimental hamsters. Oral administration of hesperetin (20 mg/kg b.w.) to DMBA painted hamsters significantly reversed the stages of oral SCC. Our findings indicate that hesperetin possesses a chemopreventive effect in DMBA-induced oral SCC by exerting anti-carcinogenic property.

Keywords: Hesperetin; antioxidants; apoptosis; detoxification agents.

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene / toxicity*
  • Animals
  • Anticarcinogenic Agents / pharmacology*
  • Antioxidants / metabolism
  • Apoptosis / drug effects
  • Blotting, Western
  • Carcinogens / toxicity
  • Carcinoma, Squamous Cell / chemically induced
  • Carcinoma, Squamous Cell / drug therapy
  • Carcinoma, Squamous Cell / pathology*
  • Cell Proliferation / drug effects
  • Cheek / pathology*
  • Citrus / chemistry*
  • Cricetinae
  • Flavanones / pharmacology*
  • Hesperidin / pharmacology*
  • Immunoenzyme Techniques
  • Lipid Peroxidation / drug effects
  • Male
  • Mesocricetus
  • Mouth Neoplasms / chemically induced
  • Mouth Neoplasms / drug therapy
  • Mouth Neoplasms / pathology*


  • Anticarcinogenic Agents
  • Antioxidants
  • Carcinogens
  • Flavanones
  • 9,10-Dimethyl-1,2-benzanthracene
  • Hesperidin
  • hesperetin
  • flavanone