In Vivo Delivery and Therapeutic Effects of a MicroRNA on Colorectal Liver Metastases

Mol Ther. 2017 Jul 5;25(7):1588-1595. doi: 10.1016/j.ymthe.2017.04.005. Epub 2017 Apr 28.

Abstract

Multiple therapeutic agents are typically used in concert to effectively control metastatic tumors. Recently, we described microRNAs that are associated with the oligometastatic state, in which a limited number of metastatic tumors progress to more favorable outcomes. Here, we report the effective delivery of an oligometastatic microRNA (miR-655-3p) to colorectal liver metastases using nanoscale coordination polymers (NCPs). The NCPs demonstrated a targeted and prolonged distribution of microRNAs to metastatic liver tumors. Tumor-targeted microRNA miR-655-3p suppressed tumor growth when co-delivered with oxaliplatin, suggesting additive or synergistic interactions between microRNAs and platinum drugs. This is the first known example of systemically administered nanoparticles delivering an oligometastatic microRNA to advanced metastatic liver tumors and demonstrating tumor-suppressive effects. Our results suggest a potential therapeutic strategy for metastatic liver disease by the co-delivery of microRNAs and conventional cytotoxic agents using tumor-specific NCPs.

Keywords: colorectal cancer; liver; metastases; microRNA; nanoparticles.

MeSH terms

  • Animals
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Cholesterol / chemistry
  • Cholesterol / metabolism
  • Colorectal Neoplasms / genetics
  • Colorectal Neoplasms / mortality
  • Colorectal Neoplasms / pathology
  • Colorectal Neoplasms / therapy*
  • Dihydroxyphenylalanine / chemistry
  • Dihydroxyphenylalanine / metabolism
  • Disease Models, Animal
  • Drug Carriers
  • Drug Synergism
  • Female
  • Gene Expression Regulation, Neoplastic*
  • HCT116 Cells
  • Humans
  • Liver Neoplasms / genetics
  • Liver Neoplasms / mortality
  • Liver Neoplasms / secondary
  • Liver Neoplasms / therapy*
  • Mice
  • Mice, Nude
  • MicroRNAs / administration & dosage
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Nanostructures / administration & dosage*
  • Nanostructures / chemistry
  • Organoplatinum Compounds / chemistry
  • Organoplatinum Compounds / pharmacology*
  • Oxaliplatin
  • Polyethylene Glycols / chemistry
  • Polyethylene Glycols / metabolism
  • Survival Analysis
  • Xenograft Model Antitumor Assays

Substances

  • Antineoplastic Agents
  • Drug Carriers
  • MIRN655 microRNA, human
  • MicroRNAs
  • Organoplatinum Compounds
  • Oxaliplatin
  • Polyethylene Glycols
  • Dihydroxyphenylalanine
  • Cholesterol
  • polyethylene glycol 2000