Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system

J Vet Med Sci. 2017 Jun 16;79(6):1061-1063. doi: 10.1292/jvms.17-0105. Epub 2017 May 1.


Equine influenza (EI) is a respiratory disease caused by equine influenza A virus (EIV, H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previously reported that some rapid antigen detection (RAD) tests are fit for diagnosing EI although their sensitivity is not optimal. Here, we evaluated the performance of the newly developed RAD test using silver amplification immunochromatography (Quick Chaser Auto Flu A, B: QCA) to diagnose EI. The detection limits of QCA for EIVs were five-fold lower than the conventional RAD tests. The duration of virus antigen detection in the infected horses was longer than the conventional RAD tests. We conclude that QCA could be a valuable diagnostic method for EI.

Keywords: diagnosis; equine influenza; rapid antigen detection; silver amplification.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Chromatography, Affinity / methods
  • Chromatography, Affinity / veterinary*
  • Horse Diseases / diagnosis*
  • Horse Diseases / virology
  • Horses
  • Influenza A Virus, H3N8 Subtype*
  • Orthomyxoviridae Infections / diagnosis
  • Orthomyxoviridae Infections / veterinary*
  • Silver
  • Time Factors


  • Silver