Cardioprotective effect of curcumin and piperine combination against cyclophosphamide-induced cardiotoxicity

Indian J Pharmacol. Jan-Feb 2017;49(1):65-70. doi: 10.4103/0253-7613.201015.

Abstract

Objective: Curcumin is a well-established cardioprotective phytoconstituent, but the poor bioavailability associated with it is always a matter of therapeutic challenge. The present study was undertaken to increase the therapeutic efficacy of curcumin by combining with bio-enhancer like piperine against cyclophosphamide (CP)-induced cardiotoxicity in rats.

Materials and methods: Rats (n = 8) were treated with curcumin (200 mg/kg, p.o.) alone and different dose combination of curcumin (100, 50, 25 mg/kg, p.o.) and piperine (20 mg/kg, p.o.) for 10 days. All the treated groups were subjected to CP (200 mg/kg, i.p.) toxicity on day 1. Twenty-four hours after the last treatment, the effects were evaluated by changes in electrocardiographic (ECG) parameters, serum biomarkers, lipid profile, tissue antioxidants, and histopathological examination. Serum and tissue homogenate parameters were measured by semi-autoanalyzer and spectrophotometer, respectively. Results obtained were assessed by one-way analysis of variance followed by Tukey-Karmer multiple comparison test.

Results: Incorporation of piperine with the doses of 50 and 25 mg/kg with curcumin exhibited significant beneficial effect compared to curcumin alone-treated group. The best effective group was a combination of curcumin 50 mg/kg with piperine 20 mg/kg which showed extremely significant (P < 0.001) decrease and increase in ECG and serum biomarker level, respectively, and moderate significant (P < 0.01) decrease in lipid profile, antioxidant levels, and histopathological score, compared to curcumin alone-treated group.

Conclusion: From this study, it can be concluded that a novel dose combination of curcumin (50 mg/kg) with piperine (20 mg/kg) exhibited profound cardioprotection compared to curcumin (200 mg/kg) alone-treated group.

Keywords: Cardioprotective; cardiotoxicity; curcumin; cyclophosphamide; piperine.

MeSH terms

  • Alkaloids / administration & dosage
  • Alkaloids / pharmacology*
  • Animals
  • Antineoplastic Agents, Alkylating
  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology
  • Benzodioxoles / administration & dosage
  • Benzodioxoles / pharmacology*
  • Biomarkers / blood
  • Cardiotonic Agents / administration & dosage
  • Cardiotonic Agents / pharmacology
  • Cardiotoxicity / etiology
  • Cardiotoxicity / prevention & control*
  • Curcumin / administration & dosage
  • Curcumin / pharmacology*
  • Cyclophosphamide / toxicity*
  • Dose-Response Relationship, Drug
  • Drug Therapy, Combination
  • Electrocardiography
  • Male
  • Piperidines / administration & dosage
  • Piperidines / pharmacology*
  • Polyunsaturated Alkamides / administration & dosage
  • Polyunsaturated Alkamides / pharmacology*
  • Rats
  • Rats, Wistar

Substances

  • Alkaloids
  • Antineoplastic Agents, Alkylating
  • Antioxidants
  • Benzodioxoles
  • Biomarkers
  • Cardiotonic Agents
  • Piperidines
  • Polyunsaturated Alkamides
  • Cyclophosphamide
  • Curcumin
  • piperine