Complete genome sequence of Bacillus velezensis LM2303, a biocontrol strain isolated from the dung of wild yak inhabited Qinghai-Tibet plateau

J Biotechnol. 2017 Jun 10:251:124-127. doi: 10.1016/j.jbiotec.2017.04.034. Epub 2017 Apr 28.

Abstract

Bacillus velezensis LM2303 is a biocontrol strain with a broad inhibitory spectrum against plant pathogens, isolated from the dung of wild yak inhabited Qinghai-Tibet plateau, China. Here we present its complete genome sequence, which consists of a single, circular chromosome of 3,989,393bp with a 46.68% G+C content. Genome analysis revealed genes encoding specialized functions for the biosynthesis of antifungal metabolites and antibacterial metabolites, the promotion of plant growth, the alleviation of oxidative stress and nutrient utilization. And the biosynthesis of antimicrobial metabolites in strain LM2303 was confirmed by biochemical analysis, while its plant growth promoting traits were confirmed by inoculation tests. Our results will establish a better foundation for further studies and biocontrol application of B. velezensis LM2303.

Keywords: Antimicrobial metabolites; Bacillus velezensis; Biocontrol; Complete genome sequence; Plant pathogens.

MeSH terms

  • Animals
  • Bacillus / genetics*
  • Biological Control Agents*
  • Cattle*
  • Feces / microbiology*
  • Genome, Bacterial*
  • Multigene Family
  • Oxidative Stress
  • Seeds / growth & development
  • Tibet
  • Triticum / growth & development

Substances

  • Biological Control Agents