dpy-13: a nematode collagen gene that affects body shape

Cell. 1988 Nov 18;55(4):567-76. doi: 10.1016/0092-8674(88)90215-2.

Abstract

Mutations in the Caenorhabditis elegans dpy-13 (dumpy) gene result in a short, chunky body shape. This gene was tagged by insertion of the Tc1 transposon, and the wild-type gene was cloned by chromosomal walking 11 kb from ama-1, a cloned gene encoding the large subunit of RNA polymerase II. Three transposon insertion sites in dpy-13 are located near the 5' end of a 1.2 kb transcribed region. The EMS-induced reference allele, dpy-13(e184), carries a small deletion near the middle of this gene. The DNA sequence reveals that dpy-13 is a member of the collagen multi-gene family, and it could encode a polypeptide of 302 amino acids. A 146 base pair sequence, encoding amino acids 56-103, is unique in the C. elegans genome, and it hybridizes to a 1 kb mRNA of moderate abundance.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • Caenorhabditis / genetics*
  • Caenorhabditis / growth & development
  • Chromosome Deletion
  • Collagen / genetics*
  • DNA / analysis
  • DNA Transposable Elements
  • Molecular Sequence Data
  • Mutation
  • RNA Polymerase II / genetics

Substances

  • DNA Transposable Elements
  • Collagen
  • DNA
  • RNA Polymerase II

Associated data

  • GENBANK/M23559