Human bronchial epithelial cells with integrated SV40 virus T antigen genes retain the ability to undergo squamous differentiation

Differentiation. 1988 Jun;38(1):60-6. doi: 10.1111/j.1432-0436.1988.tb00592.x.


Human bronchial epithelial cells transformed by either DNA virus infection (SV40 or Adenovirus 12-SV40 hybrid virus) or transfection with the SV40 large T antigen gene were studied for their ability to undergo squamous differentiation when exposed to 12-O-tetradecanoylphorbol-13 acetate (TPA), transforming growth factor-beta 1 (TGF-beta 1), or fetal bovine serum (FBS), agents that induce the squamous differentiation of normal human bronchial epithelial cells. Squamous differentiation occurred in all ten T-antigen-positive cell cultures when they were exposed to either FBS or TGF-beta 1, but none differentiated when exposed to TPA. From one cell line, designated BEAS-2B, two subclones were isolated, one of which was induced to undergo squamous differentiation by FBS, and a second that failed to undergo squamous differentiation and was mitogenically stimulated when exposed to serum. These phenotypically different subclones provide a new in vitro cellular system for delineating the mechanism(s) of human bronchial epithelial cell squamous differentiation in response to FBS or TGF-beta 1.

MeSH terms

  • Antigens, Viral / metabolism*
  • Bronchi / cytology*
  • Bronchi / drug effects
  • Bronchi / immunology
  • Cell Differentiation
  • Cell Line
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Humans
  • Parainfluenza Virus 1, Human / immunology*
  • Serum Albumin, Bovine / pharmacology
  • Tetradecanoylphorbol Acetate / analogs & derivatives
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transforming Growth Factors / pharmacology
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / immunology*


  • Antigens, Viral
  • Serum Albumin, Bovine
  • 12-O-tetradeca-2,4,6,8-tetranoylphorbol-13-acetate
  • Transforming Growth Factors
  • Tetradecanoylphorbol Acetate