Presence of phosphatidylcholine hydroperoxide in human plasma

J Biochem. 1988 May;103(5):744-6. doi: 10.1093/oxfordjournals.jbchem.a122339.

Abstract

A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cytochrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.

MeSH terms

  • Calibration
  • Chromatography, High Pressure Liquid
  • Cytochrome c Group
  • Female
  • Humans
  • Luminescent Measurements
  • Luminol
  • Oxidation-Reduction
  • Phosphatidylcholines / blood*

Substances

  • Cytochrome c Group
  • Phosphatidylcholines
  • phosphatidylcholine hydroperoxide
  • Luminol