DNA hybridization assay for congenital cytomegalovirus infection

J Clin Microbiol. 1988 Oct;26(10):2184-6. doi: 10.1128/jcm.26.10.2184-2186.1988.


An in vitro DNA hybridization assay was used to test 281 newborns for congenital infection with cytomegalovirus. The assay utilized an abbreviated method for DNA preparation and a dot blot assay that provided good sensitivity (100%) and specificity (98.9%) when compared with standard tissue culture, yet substantially reduced the total time of analysis. This assay would be a useful adjunct to tissue culture to diagnose newborns with congenital infection with cytomegalovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytomegalovirus Infections / congenital
  • Cytomegalovirus Infections / diagnosis*
  • DNA, Viral / urine*
  • Humans
  • Infant, Newborn
  • Nucleic Acid Hybridization*


  • DNA, Viral