Proteome profile of spinneret from the silkworm, Bombyx mori

Proteomics. 2017 Jun;17(12). doi: 10.1002/pmic.201600301.

Abstract

The silkworm spinneret is an important tissue for silk fibrillogenesis and spinning. All biochemical processes during silk fibrillogenesis are correlated with silk properties. Understanding the role of spinneret in silk fibrillogenesis may help to reveal the mechanism of silk fibrillogenesis as well as improve silk quality for commercial purposes. Thus, we profiled the proteome of silkworm spinneret. A total of 1572 proteins and 232 differential abundance proteins were identified. Silk fibrillogenesis-related proteins, such as cuticle proteins, ion-transporting proteins, muscular proteins, and energy metabolic proteins, were abundant in spinneret. Metabolic pathway and GO enrichment analyses revealed that the identified proteins were involved in energy metabolism, chitin binding, and cuticle construction. Active energy metabolism may provide abundant energy for the muscle contraction as well as ion and water exchange. The chitin binding and cuticle construction process may provide sufficient shear forces for silk formation. Our data suggest that silkworm spinneret provides a suitable physiological and biochemical environment for silk fibrillogenesis. These proteins are potential targets for improving silk quality in the silk industry. Data are available via ProteomeXchange with identifier PXD004455.

Keywords: Cuticle proteins; Insect proteomics; Ion-transporting proteins; LC-MS/MS; Silk fibrillogenesis.

MeSH terms

  • Animals
  • Bombyx / chemistry*
  • Insect Proteins / analysis*
  • Proteome / analysis*
  • Proteomics
  • Silk / analysis*

Substances

  • Insect Proteins
  • Proteome
  • Silk