The reduction in inflammation and impairment in wound healing by using strontium chloride hexahydrate

Cutan Ocul Toxicol. 2018 Mar;37(1):24-28. doi: 10.1080/15569527.2017.1326497. Epub 2017 May 22.


Background: Numerous growth factors, cytokine, mitogen and chemotactic factors are involved in wound healing. Even though inflammation is important for the stimulation of proliferative phase, excessive inflammation also causes impairment in wound healing. Strontium salts suppress keratinocyte-induced TNF-alpha and interleukin-1 and interleukin-6 in in vitro cultures. This study was conducted to determine the effects of administration of topical strontium chloride hexahydrate on wound healing through TNF-alpha and TGF-beta in surgical wound healing model of in-vivo rat skin.

Material and methods: Twenty-four rats were used in the study. After approximately 2 cm cutaneous-subcutaneous incision was horizontally carried out on the mid-neckline of the rats, the incision was again closed using 2.0 vicryl. The rats were assigned into three groups including eight rats in each group. Placebo emollient ointment and also the ointments, which were containing 5% and 10% strontium chloride hexahydrate and were prepared at the same base with placebo ointment, were administered to the groups by a blind executor twice a day for a week. At the end of seventh day, the rats were sacrificed and cutaneous and subcutaneous tissue of their wound site was resected for histopathological examination. Scoring of histopathological wound healing and scoring of tissue TNF-alpha and TGF-beta level with immunohistochemical staining were performed.

Results: The groups, to which both 5% and 10% strontium chloride hexahydrate was administered, had lower immunohistochemical TNF-alpha levels and histopathological wound scores compared to controls, which was statistically significant (p < 0.05).

Conclusion: Strontium chloride hexahydrate can lead to impairment in wound healing by suppressing inflammation through TNF-alpha.

Keywords: Inflammation; TNF-alpha; strontium; wound; wound healing.

MeSH terms

  • Administration, Cutaneous
  • Animals
  • Dermatologic Surgical Procedures
  • Female
  • Inflammation / immunology
  • Rats, Wistar
  • Skin / drug effects
  • Skin / immunology
  • Skin / pathology
  • Strontium / pharmacology*
  • Transforming Growth Factor beta / immunology
  • Tumor Necrosis Factor-alpha / immunology*
  • Wound Healing / drug effects*


  • Transforming Growth Factor beta
  • Tumor Necrosis Factor-alpha
  • strontium chloride
  • Strontium