Ovariectomy uncovers purinergic receptor activation of endothelin-dependent natriuresis

Am J Physiol Renal Physiol. 2017 Aug 1;313(2):F361-F369. doi: 10.1152/ajprenal.00098.2017. Epub 2017 May 3.


We recently reported that natriuresis produced by renal medullary salt loading is dependent on endothelin (ET)-1 and purinergic (P2) receptors in male rats. Because sex differences in ET-1 and P2 signaling have been reported, we decided to test whether ovarian sex hormones regulate renal medullary ET-1 and P2-dependent natriuresis. The effect of medullary NaCl loading on Na+ excretion was determined in intact and ovariectomized (OVX) female Sprague-Dawley rats with and without ET-1 or P2 receptor antagonism. Isosmotic saline (284 mosmol/kgH2O) was infused in the renal medullary interstitium of anesthetized rats during a baseline urine collection period, followed by isosmotic or hyperosmotic saline (1,800 mosmol/kgH2O) infusion. Medullary NaCl loading significantly enhanced Na+ excretion in intact and OVX female rats. ETA+B or P2 receptor blockade did not attenuate the natriuretic effect of medullary NaCl loading in intact females, whereas ETA+B or P2 receptor blockade attenuated the natriuretic response to NaCl loading in OVX rats. Activation of medullary P2Y2 and P2Y4 receptors by UTP infusion had no significant effect in intact females but enhanced Na+ excretion in OVX rats. Combined ETA+B receptor blockade significantly inhibited the natriuretic response to UTP observed in OVX rats. These data demonstrate that medullary NaCl loading induces ET-1 and P2-independent natriuresis in intact females. In OVX, activation of medullary P2 receptors promotes ET-dependent natriuresis, suggesting that ovarian hormones may regulate the interplay between the renal ET-1 and P2 signaling systems to facilitate Na+ excretion.

Keywords: endothelin-1; purinergic receptors; renal medulla; sodium excretion.

MeSH terms

  • Animals
  • Endothelin Receptor Antagonists / pharmacology
  • Endothelin-1 / genetics
  • Endothelin-1 / metabolism*
  • Female
  • Kidney Medulla / drug effects
  • Kidney Medulla / metabolism*
  • Natriuresis* / drug effects
  • Ovariectomy*
  • Purinergic P2 Receptor Agonists / pharmacology
  • Purinergic P2 Receptor Antagonists / pharmacology
  • Rats, Sprague-Dawley
  • Receptors, Purinergic P2 / drug effects
  • Receptors, Purinergic P2 / metabolism*
  • Receptors, Purinergic P2Y2 / drug effects
  • Receptors, Purinergic P2Y2 / metabolism*
  • Renal Elimination* / drug effects
  • Signal Transduction
  • Sodium / urine*
  • Sodium Chloride / administration & dosage
  • Sodium Chloride / metabolism
  • Time Factors


  • Endothelin Receptor Antagonists
  • Endothelin-1
  • P2ry2 protein, rat
  • Purinergic P2 Receptor Agonists
  • Purinergic P2 Receptor Antagonists
  • Receptors, Purinergic P2
  • Receptors, Purinergic P2Y2
  • purinoceptor P2Y4
  • Sodium Chloride
  • Sodium