Developmental competence of porcine genome-edited zygotes

Mol Reprod Dev. 2017 Sep;84(9):814-821. doi: 10.1002/mrd.22829. Epub 2017 Jun 8.


Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases-including ZFNs, TALENs, and the CRISPR/Cas9 system-has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.

Keywords: CRISPR/Cas9; TALEN; ZFN; cytoplasmic microinjection; in vitro-produced zygotes; in vivo-derived zygotes.

Publication types

  • Review

MeSH terms

  • Animals
  • Animals, Genetically Modified / embryology*
  • Animals, Genetically Modified / genetics*
  • CRISPR-Cas Systems*
  • Gene Editing / methods*
  • Swine
  • Zygote / cytology
  • Zygote / metabolism*