The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension

Cell. 2017 May 4;169(4):693-707.e14. doi: 10.1016/j.cell.2017.04.013.


The spatial organization of chromosomes influences many nuclear processes including gene expression. The cohesin complex shapes the 3D genome by looping together CTCF sites along chromosomes. We show here that chromatin loop size can be increased and that the duration with which cohesin embraces DNA determines the degree to which loops are enlarged. Cohesin's DNA release factor WAPL restricts this loop extension and also prevents looping between incorrectly oriented CTCF sites. We reveal that the SCC2/SCC4 complex promotes the extension of chromatin loops and the formation of topologically associated domains (TADs). Our data support the model that cohesin structures chromosomes through the processive enlargement of loops and that TADs reflect polyclonal collections of loops in the making. Finally, we find that whereas cohesin promotes chromosomal looping, it rather limits nuclear compartmentalization. We conclude that the balanced activity of SCC2/SCC4 and WAPL enables cohesin to correctly structure chromosomes.

Keywords: CTCF; MAU2; NIPBL; SCC2; SCC4; TADs; WAPL; chromatin looping; cohesin; loop extrusion.

MeSH terms

  • Acetyltransferases / metabolism
  • CCCTC-Binding Factor
  • Carrier Proteins / metabolism*
  • Cell Cycle Proteins / metabolism
  • Cell Nucleus / metabolism*
  • Chromatin / metabolism*
  • Chromosomal Proteins, Non-Histone / metabolism
  • Cohesins
  • DNA-Binding Proteins
  • Fatty Acid Elongases
  • Gene Editing
  • Humans
  • Multiprotein Complexes / metabolism
  • Nuclear Proteins / metabolism*
  • Proto-Oncogene Proteins / metabolism*
  • Repressor Proteins / metabolism


  • CCCTC-Binding Factor
  • CTCF protein, human
  • Carrier Proteins
  • Cell Cycle Proteins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • MAU2 protein, human
  • Multiprotein Complexes
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • WAPL protein, human
  • Acetyltransferases
  • Fatty Acid Elongases