RNA editing of cytochrome c maturation transcripts is responsive to the energy status of leaf cells in Arabidopsis thaliana

Yuzhe Sun; Yee-Song Law; Shifeng Cheng; Boon Leong Lim

doi:10.1016/j.mito.2017.04.006

RNA editing of cytochrome c maturation transcripts is responsive to the energy status of leaf cells in Arabidopsis thaliana

Mitochondrion. 2017 Jul:35:23-34. doi: 10.1016/j.mito.2017.04.006. Epub 2017 May 3.

Authors

Yuzhe Sun¹, Yee-Song Law¹, Shifeng Cheng¹, Boon Leong Lim²

Affiliations

¹ School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China.
² School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China; State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong, China. Electronic address: bllim@hku.hk.

PMID: 28478183
DOI: 10.1016/j.mito.2017.04.006

Abstract

Overexpression of AtPAP2, a phosphatase located on the outer membranes of chloroplasts and mitochondria, leads to higher energy outputs from these organelles. AtPAP2 interacts with seven MORF proteins of the editosome complex. RNA-sequencing analysis showed that the editing degrees of most sites did not differ significantly between OE and WT, except some sites on the transcripts of several cytochrome c maturation (Ccm) genes. Western blotting of 2D BN-PAGE showed that the patterns of CcmF_N1 polypeptides were different between the lines. We proposed that AtPAP2 may influence cytochrome c biogenesis by modulating RNA editing through its interaction with MORF proteins.

Keywords: AtPAP2; Ccm; Cytochrome c; MORF; RNA editing.

MeSH terms

Arabidopsis / genetics*
Arabidopsis / metabolism*
Cytochromes c / biosynthesis*
Cytochromes c / genetics
Energy Metabolism*
RNA Editing*
Transcription Factors / genetics*
Transcription Factors / metabolism*

Substances

PAP2 protein, Arabidopsis
Transcription Factors
Cytochromes c