Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules

EMBO J. 1988 Aug;7(8):2321-7.

Abstract

Previous genetic studies have shown that the fission yeast cdc13+ gene product interacts closely with the cdc2+ protein kinase during mitosis. Here, we have cloned the cdc13+ gene from a S. pombe gene bank by complementation of the temperature-sensitive defect of a cdc13-117 mutant strain. The complementing activity was localized to a 1.9-kb XbaI-NsiI DNA fragment, and nucleotide sequencing revealed a 1446-bp open reading frame. The predicted amino acid sequence contained 482 residues and was not homologous to any protein in a protein database. The cdc13+ gene function was confirmed to be essential for cell division since cells carrying a cdc13 null allele arrested with a cdc phenotype. However, unlike any existing temperature-sensitive cdc13 mutants, cdc13 null mutants arrested in G2 without septa or condensed chromosomes indicating that cdc13+ gene function is required at or prior to the initiation of mitotis. cdc13-117 mutant strains were found to be hypersensitive to the tubulin inhibitor thiabendazole. This observation suggests that the cdc13+ gene product, which is required for mitotic initiation, may interact with microtubules.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Genes, Fungal
  • Genetic Complementation Test
  • Microtubules / metabolism*
  • Mitosis*
  • Molecular Sequence Data
  • Mutation
  • Plasmids
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • Saccharomycetales / genetics*
  • Schizosaccharomyces / drug effects
  • Schizosaccharomyces / enzymology
  • Schizosaccharomyces / genetics*
  • Thiabendazole / pharmacology
  • Transformation, Genetic

Substances

  • Protein Kinases
  • DNA Restriction Enzymes
  • Thiabendazole