Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):18-21. doi: 10.21010/ajtcam.v13i6.4. eCollection 2016.


Background: The current trend globally is the utilization of natural products as therapeutic agents given its minimum side effects. The leaves of Stevia contain several active ingredient compounds such as rebaudioside. Stevia extract have been used for many purposes. Active oxygen radicals can induce base modifications, DNA breakage, and intracellular protein crosslink's. This study was done to evaluate the potential of stevia extract as antibacterial and antioxidants actions.

Materials and methods: Antibacterial activity of different extracts of stevia was tested in vitro against different species of bacteria and hepato-protective efficacy was testes in rats injected with CCl4 as hepatotoxic.

Results: Acetone extract exhibited antibacterial activity against selected five bacteria species. The acetone extract suppressed the elevation of serum ALT (p <0.05) and AST (p <0.001) activities induced by CCl4. Animals given stevia extract showed prevention against deleterious effects of CCl4 by lowering lipid peroxidation and enhancement of antioxidant activities as SOD and CAT. The protection trial is better than treatment trial. Total phenolic content of aqueous and acetone extracts were found 30 mg and 85 mg gallic /gm extract respectively. While the total flavonoids were 40 mg and 80 mg quercetin/g respectively. The GC-MS analysis showed that monoterpene and indole are the main components. Aqueous extract don't show any antibacterial activity against the tested strains. The antioxidant properties were attributable to its phenolic content to scavenge free radicals.

Conclusion: Acetone extract possess a potent antimicrobial and activity against deleterious effect of CCl4-caused liver damage.

Keywords: antibacterial; antioxidant enzymes; hepatic damage; stevia.

MeSH terms

  • Acetone / pharmacology
  • Alanine Transaminase / blood
  • Animals
  • Anti-Infective Agents / pharmacology*
  • Antioxidants / pharmacology*
  • Aspartate Aminotransferases / blood
  • Carbon Tetrachloride Poisoning / blood
  • Carbon Tetrachloride Poisoning / drug therapy
  • Carbon Tetrachloride Poisoning / etiology
  • Catalase / drug effects
  • Flavonoids / analysis
  • Free Radical Scavengers / pharmacology
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Male
  • Phenols / analysis
  • Phytotherapy / methods*
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Rats
  • Stevia / chemistry*
  • Superoxide Dismutase / drug effects


  • Anti-Infective Agents
  • Antioxidants
  • Flavonoids
  • Free Radical Scavengers
  • Phenols
  • Plant Extracts
  • Acetone
  • Catalase
  • Superoxide Dismutase
  • Aspartate Aminotransferases
  • Alanine Transaminase