Super-resolution optical microscopy for studying membrane structure and dynamics

J Phys Condens Matter. 2017 Jul 12;29(27):273001. doi: 10.1088/1361-648X/aa7185. Epub 2017 May 8.


Investigation of cell membrane structure and dynamics requires high spatial and temporal resolution. The spatial resolution of conventional light microscopy is limited due to the diffraction of light. However, recent developments in microscopy enabled us to access the nano-scale regime spatially, thus to elucidate the nanoscopic structures in the cellular membranes. In this review, we will explain the resolution limit, address the working principles of the most commonly used super-resolution microscopy techniques and summarise their recent applications in the biomembrane field.

Publication types

  • Review

MeSH terms

  • Cell Membrane*
  • Microscopy / methods*
  • Microscopy, Confocal / methods
  • Microscopy, Fluorescence / methods