Electrochemical switching with a DNA aptamer-based electrochemical sensor

Shabnam Beiranvand; Azadeh Azadbakht

doi:10.1016/j.msec.2017.03.028

Electrochemical switching with a DNA aptamer-based electrochemical sensor

Mater Sci Eng C Mater Biol Appl. 2017 Jul 1:76:925-933. doi: 10.1016/j.msec.2017.03.028. Epub 2017 Mar 6.

Authors

Shabnam Beiranvand¹, Azadeh Azadbakht²

Affiliations

¹ Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
² Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran. Electronic address: Azadbakht.a@gmail.com.

PMID: 28482608
DOI: 10.1016/j.msec.2017.03.028

Abstract

The present study was focused on the application of NH₂-functionalized Fe₃O₄/gold nanoparticles (Fe₃O₄/AuNPs)-decorated carbon nanotubes (CNTs) in the development of electrochemical sensor for bisphenol A (BPA) detection. After the nanocomposite synthesis and its characterization, the optimization of the measurement conditions and working parameters of sensors were evaluated. Aminated detection probe (DNA aptamer) was surface confined on the NH₂-functionalized Fe₃O₄/AuNPs surface using glutaraldehyde as a linker. The constructed nanoaptasensor incorporated the advantages of the neatly deposited Fe₃O₄/AuNPs and the covalent attachment of the detection probe at the surface of sensing interface. The results revealed that BPA could be detected in a wide linear range from 1 to 600nM with a low detection limit down to 300pM. Moreover, the resultant aptasensor exhibited good specificity, stability and reproducibility, indicating that the present strategy was promising for broad potential application in clinic assay.

Keywords: Aptasensor; Bisphenol A; Carbon nanotube; NH(2)-functionalized Fe(3)O(4).

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques
Electrochemical Techniques
Gold
Metal Nanoparticles
Nanotubes, Carbon
Reproducibility of Results

Substances

Aptamers, Nucleotide
Nanotubes, Carbon
Gold