SOD2 gene polymorphism and response of oxidative stress parameters in young wrestlers to a three-month training

Free Radic Res. 2017 May;51(5):506-516. doi: 10.1080/10715762.2017.1327716.

Abstract

The aim of the study was to analyse the effect of Val 16Ala polymorphism in SOD2 gene on oxidative stress parameters and lipid profile of the blood during a three-month wrestling training. The study included 53 Polish young wrestlers. Blood samples were collected at the beginning of the programme and following three months of the training. The list of analysed parameters included erythrocyte and serum activities of superoxide dismutase (SOD), whole blood glutathione peroxidase (GPx) activity, total glutathione (tGSH) level, concentration of lipid hydroperoxides (LHs), total antioxidant capacity (TAC) and creatine kinase (CK) activity in the serum, as well as lipid profile parameters: triglycerides (TG), total cholesterol (TC), high-density (HDL-C), and low-density lipoprotein cholesterol (LDL-C). Three-month training resulted in a decrease in CK activity, an increase in serum SOD activity, as well as in unfavourable changes in serum lipid profile: an increase in TC, LDL-C, and TG, and a decrease in HDL-C. Aside from CK activity, all these changes seemed to be associated with presence of Val allele. Prior to the training programme, subjects with Ala/Ala genotype presented with lower levels of LHs, lower whole blood GPx activity, and lower serum concentrations of TC than the individuals with Ala/Val genotype. Both prior to and after three-month training, higher levels of tGSH were observed in Val/Val genotype as compared to Ala/Val genotype carriers. Moreover, multiple regression analysis demonstrated that SOD2 genotype was a significant predictor of pre-training whole blood GPx activity and erythrocyte SOD activity (Val/Val > Ala/Val > Ala/Ala). Altogether, these findings suggest that Val 16Ala polymorphism in SOD2 gene contributes to individual variability in oxidative stress status and lipid profile of the blood in young wrestlers, and may modulate biochemical response to training.

Keywords: SOD2; gene polymorphism; oxidative stress; wrestlers; wrestling training.

MeSH terms

  • Adolescent
  • Cholesterol, LDL / blood
  • Erythrocytes / enzymology
  • Exercise
  • Genetic Association Studies
  • Genetic Linkage
  • Humans
  • Male
  • Oxidative Stress
  • Polymorphism, Single Nucleotide
  • Superoxide Dismutase / blood
  • Superoxide Dismutase / genetics*
  • Triglycerides / blood
  • Wrestling

Substances

  • Cholesterol, LDL
  • Triglycerides
  • Superoxide Dismutase
  • superoxide dismutase 2