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Significantly Enhanced Biomass Production of a Novel Bio-Therapeutic Strain Lactobacillus plantarum (AS-14) by Developing Low Cost Media Cultivation Strategy

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Significantly Enhanced Biomass Production of a Novel Bio-Therapeutic Strain Lactobacillus plantarum (AS-14) by Developing Low Cost Media Cultivation Strategy

Asma Manzoor et al. J Biol Eng.

Abstract

Background: Probiotic bacteria are becoming an important tool for improving human health, controlling diseases and enhancing immune responses. The availability of a cost effective cultivation conditions has profound effect on the efficiency and role of probiotic bacteria. Therefore the current study was conducted with an objective to develop a low cost growth medium for enhancing the biomass production of a bio-therapeutic bacterial strain Lactobacillus plantarum AS-14. In this work the isolation of Lactobacillus plantarum AS-14 bacterial strain was carried out from brinjal using cheese whey as a main carbon source. Moreover, the effect of four other nutritional factors besides cheese whey was investigated on the enhanced cell mass production by using response surface methodology (RSM).

Results: The best culture medium contained 60 g/l cheese whey, 15 g/l glucose and 15 g/l corn steep liquor in addition to other minor ingredients and it resulted in maximum dry cell mass (15.41 g/l). The second-order polynomial regression model determined that the maximum cell mass production (16.02 g/l) would be obtained at temperature 40°C and pH 6.2. Comparative studies showed that cultivation using cheese whey and corn steep liquor with other components of the selected medium generated higher biomass with lower cost than that of De Man, Rogosa and Sharpe (MRS) medium under similar cultivation conditions (pH 6.2 and temperature 40°C).

Conclusion: It is evident that the cell biomass of L. Plantarum AS-14 was enhanced by low cost cultivation conditions. Moreover, corn steep liquor and ammonium bisulphate were perceived as low-cost nitrogen sources in combination with other components to substitute yeast extract. Of all these factors, cheese whey, corn steep liquor, yeast extract and two operating conditions (temperature and pH) were found to be the most significant parameters. Thus the cost effective medium developed in this research might be used for large-scale commercial application where economics is quite likely important.

Keywords: Cheese whey; Lactobacillus plantarum; Medium optimization; Nitrogen sources; Response surface methodology.

Figures

Fig. 1
Fig. 1
Response surface of dry cell mass production by Lactobacillus plantarum (AS-14), showing the interaction of glucose (X1) and Cheese whey (X3) at constant levels of corn steep liquor (15 g/l) yeast extract (5 g/l), sodium acetate (5 g/l), MgSO4 7H2O (0.3 g/l), and MnSO4 4H2O (0.04 g/l)
Fig. 2
Fig. 2
Response surface of dry cell mass production by Lactobacillus plantarum (AS-14), showing the interaction of glucose (X1) and corn steep liquor (X5) at constant levels of cheese whey (60 g/l) yeast extract (5 g/l), Sodium acetate (5 g/l), MgSO4 7H2O (0.3 g/l), and MnSO4 4H2O (0.04 g/l)
Fig. 3
Fig. 3
Response surface plot in relation to temperature and pH for dry mass production
Fig. 4
Fig. 4
Comparison of dry cell mass and viable counts of Lactobacillus plantarum (AS-14) cultivated in MS medium and the optimum medium. Fermentation conditions: 5% inoculum, 40 °C, 24 h, at 100 ml medium/250 ml Hinton flask without shaking. Each value represents the mean columns are significantly different (p < 0.05) compared to MRS medium

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