Characterization of OXA-48-like-producing Enterobacteriaceae isolated from river water in Algeria

Rima Tafoukt; Abdelaziz Touati; Thongpan Leangapichart; Sofiane Bakour; Jean-Marc Rolain

doi:10.1016/j.watres.2017.04.073

Characterization of OXA-48-like-producing Enterobacteriaceae isolated from river water in Algeria

Water Res. 2017 Sep 1:120:185-189. doi: 10.1016/j.watres.2017.04.073. Epub 2017 Apr 30.

Authors

Rima Tafoukt¹, Abdelaziz Touati², Thongpan Leangapichart³, Sofiane Bakour³, Jean-Marc Rolain⁴

Affiliations

¹ Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, 06000 Bejaia, Algeria; Unité de Recherche sur les Maladies Infectieuses et Tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
² Laboratoire d'Ecologie Microbienne, FSNV, Université de Bejaia, 06000 Bejaia, Algeria.
³ Unité de Recherche sur les Maladies Infectieuses et Tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
⁴ Unité de Recherche sur les Maladies Infectieuses et Tropicales émergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France. Electronic address: jean-marc.rolain@univ-amu.fr.

PMID: 28486169
DOI: 10.1016/j.watres.2017.04.073

Abstract

The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a significant problem for healthcare worldwide. The prevalence of carbapenem-resistant Enterobacteriaceae (CPE) in water environments in Algeria are unknown. The aim of this study was to screen for the presence of CPE isolates in the Soummam River in Bejaia, Algeria. Isolates of Enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. The isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Isolates were subjected to antimicrobial susceptibility testing and the modified Carba NP test. Carbapenemase and extended-spectrum β-lactamase (ESBL) determinants were studied by PCR amplification and sequencing. The clonal relatedness between isolates was studied by Multilocus Sequence Typing (MLST) method. A total of 20 carbapenem-resistant Enterobacteriaceae strains were included in this study, identified as Escherichia coli (n = 12), Klebsiella pneumoniae (n = 3), Raoultella ornithinolytica (n = 3), Citrobacter freundii (n = 1) and Citrobacter braakii (n = 1). Carbapenemase genes identified in this study included bla_OXA-48, observed in 17 isolates (9 E. coli, 3 K. pneumoniae, 3 R. ornithinolytica, 1 C. freundii and 1 C. braakii), and bla_OXA-244, a variant of bla_OXA-48, was found in three E. coli isolates. MLST showed that 12 E. coli strains belonged to six different sequence types (ST559, ST38, ST212, ST3541, 1972 and ST2142), and we identified three different STs in K. pneumoniae isolates, including ST133, ST2055, and a new sequence type: ST2192. This study showed the presence of OXA-48-like-producing Enterobacteriaceae in water environments and highlighted the potential role of aquatic environments as reservoirs of clinically relevant antimicrobial-resistant bacteria, with the potential to spread throughout the community.

Keywords: Algeria; Enterobacteriaceae; MLST; OXA-48-like; Soummam river.

MeSH terms

Algeria
Anti-Bacterial Agents
Bacterial Proteins
Enterobacteriaceae*
Escherichia coli
Microbial Sensitivity Tests
Multilocus Sequence Typing
Oxytocin / metabolism*
Rivers*
beta-Lactamases

Substances

Anti-Bacterial Agents
Bacterial Proteins
Oxytocin
beta-Lactamases