The influence of folic acid on the picrotoxin-sensitive site of the GABAa-receptor complex

Epilepsy Res. May-Jun 1988;2(3):215-8. doi: 10.1016/0920-1211(88)90058-7.

Abstract

The site of action responsible for the convulsive effect of folic acid was investigated in vitro. Folic acid (ECmax 5 x 10(-4) M) enhances the binding of the cage convulsant [3H]t-butylbicycloorthobenzoate ([3H]TBOB) to rat brain membranes, namely to 130% of control in the absence of GABA and to over 300% of control in the presence of physiological concentrations of GABA. Analysis of the binding parameters reveals that folic acid increases the apparent number of [3H]TBOB binding sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding, Competitive
  • Bridged Bicyclo Compounds / metabolism
  • Bridged Bicyclo Compounds, Heterocyclic*
  • Convulsants / metabolism*
  • Folic Acid / metabolism*
  • Male
  • Picrotoxin / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Receptors, GABA-A / drug effects
  • Receptors, GABA-A / metabolism*

Substances

  • Bridged Bicyclo Compounds
  • Bridged Bicyclo Compounds, Heterocyclic
  • Convulsants
  • Receptors, GABA-A
  • Picrotoxin
  • Folic Acid
  • tert-butylbicyclo-2-benzoate